Accelerated Buffer System for Amino Acid Analysis

The continual increase in sample numbers in busy labs means that it is often difficult for quality control or contract analysis labs to maintain short turnaround times, particularly when instruments are already running at full capacity. To address the need for faster analysis while retaining the quality of separation offered by dedicated amino acid analysers, an improved formulation of sodium citrate based buffers has been developed by Biochrom.

Instrumentation

The Biochrom 30 amino acid analyser is a compact bench top, PC-controlled liquid chromatography system for the specific analysis of amino acids for the pharmaceutical, proteomics, food and feedstuffs industries.

Amino acids are separated by ion exchange chromatography and quantified using dual wavelength photometric detection following ninhydrin post column derivatization, as required by the AOAC method and the Commission Directive 98/64/EC. Because of the quality of the separation, high reproducibility of retention time and peak area, and the extensive stability of calibration, dedicated amino acid analysis has become the standard method for amino acid analysis of feeds.

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Author(s): 
Aurélie Lolia
Journal: 
LCGC Europe, 02 December 2007