Validation of a Selective Method for Determination of Paroxetine in human plasma by LC-MS/MS

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Purpose. A sensitive, robust, and selective liquid chromatographic – tandem mass spectrometric method (LC-MS/MS) was developed and validated for paroxetine quantification in human EDTA plasma. Methods. Sample preparation was based on liquid-liquid extraction using a mixture of ethyl acetate/hexane (50/50; v/v) to extract the drug and internal standard from plasma. Chromatography was performed on a C-18 analytical column and the retention times were 1.6 and 1.7 for paroxetine and fluoxetine (IS), respectively.

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Author(s): 
Massaroti, P.; Cassiano, N. M.; Duarte, L. F.; Campos, D. R.; Marchioretto, M. A. M.; Bernasconi, G.; Calafatti, S.; Barros, F. A. P.; Meurer, E.C.; Pedrazzoli,
Journal: 
J Pharm Pharmaceut Sci (www.cspscanada.org) 8(2) May - August 2005
Copyright: 
Copyright © by the Canadian Society for Pharmaceutical Sciences