Autoclaving is one of the technique used in moist heat sterilization. The equipment used is called as Autoclave.
The principle behind the working of an Autoclave is that- The higher the pressure created inside the autoclave, the higher would be the attainable temperature inside it.
The boiling point of water at normal atmospheric pressure is 100oC.
When the free-flowing steam at a temperature of 100oC is subjected under pressure of 1 atmosphere above the sea-level pressure i.e., 15 pounds pressure per square inch, the temperature inside the autoclave happens to rise up to 121oC, which is an usual and common parameters employed in the moist heat sterilization.
Ref:- Pg:207; Pharmaceutical Microbiology by Ashutosh Kar.
Viswanadha Institute of Pharmaceutical Sciences.
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