The chemical tests for presence of digitoxose are Legal test:To the extract in pyridine, sodium nitroprusside is added to make it alkaline.Pink or red color is produced. Baljet test:To a section of digitalis sodium picrate is added to produce yellow to orange color. Keller-Kiliani test:1g powder in 10ml 70% alcohol is boiled for 2-3 min.5ml water and 5ml strong leadacetate solution is added to the filtrate.The clear filtrate is treated with equal volume of chloroform and evaporated.The extrace is dissolved in glacial acetic acid and on cooling 2drops of ferric chloride is added.These contents are transferred to 2ml conc.sulphuric acid.A reddish brown layer acquires bluish green coplor appears due to presence of digitoxose. REF:PHARMACOGNOSY-NEERALI PRAKASHAN PUBLICATIONS.34TH EDITION, Page-197

hello, digitoxes is a cadiac sugar so you can do cardiac glycosides test for conformation of digitosxes in your plant matrial. thanks

These are characteristic for the 5-mambered lactone ring of cardenolides: 1- Kedde's test: card. + Kedde's reagent (3,5- dinitrobenzoic acid + NaOH - violet colour. 2- Baljet's test: card. +Baljet's reagent (picric acid +NaOH) - orange or red