The DNA microchip is a new tool used to identify mutations in genes. The chip, which consists of a small glass plate encased in plastic, is manufactured somewhat like a computer microchip. On the surface, each chip contains thousands of short, synthetic, single-stranded DNA sequences, which together add up to the normal gene in question.
1) Introduction
Nucleic acid hybridization, where two complementary single-stranded nucleic acid molecules form a stable hybrid has been the basis of the advances made in molecular genetics.
The two major techniques involved are Southern and Northern blotting. The former identifies specific DNA sequences in a sample, and the latter the presence of specific RNA molecules. Both of these procedures have been extremely useful, but they have technical deficiencies.