Capillary Electrophoresis Analysis of Propranolol in Human Serum Using Dynamic Capillary Coating

A capillary electrophoresis method using the CElixir buffer system for the analysis of propranolol in human serum was compared with a method using phosphate buffer. While both methods showed good linearity over the concentration range tested, the CElixir method showed better accuracy and precision at the low concentration end (25–50 ng/mL). The limits of quantitation and detection of the CElixir method were 25 and 12.5 ng/mL, respectively. Improved migrationtime reproducibility was obtained with the CElixir method, which was probably responsible for an enhanced overall performance compared with the standard phosphate method.

Introduction :
Capillary electrophoresis (CE) is gaining wider acceptance in analytical laboratories and has become an important tool for routine analysis in the pharmaceutical and biotech industries1. Specifically in the area of bioanalytical chemistry several publications report the use of CE for the analysis of drugs in biological matrices. The applications include drug quantifications for pharmacokinetic and metabolism studies,2,3 in vitro drug metabolism studies,4,5 as well as applications in forensic analysis6 and therapeutic drug monitoring.7 Methods can be validated and show good accuracy and precision.8,9

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