Structure and Dynamics of Lipoplex Formation Examined Using Two-Photon Fluoresce

While viral gene therapy possesses promising clinical application, with high transfection efficiencies, the possibility of unwanted immunological responses and mutagenesis has stimulated investigations of nonviral gene delivery methods. The dominant nonviral delivery vehicles for gene therapy are polymers and cationic lipids. Favorable electrostatic interactions drive complex formation between the DNA and lipids or polymers to form lipoplexes and polyplexes, respectively. Using appropriate lipoplex assemblies, the charged surface of the cationic lipid vesicles also ensures interaction with the negatively charged target cell surface. This step is critical for successful cellular entry of the lipoplex, which is the initial step in transfection.

For full article Click Here