HPLC and GC Articles

In the world of practical chromatography, peak shapes only approach an ideal Gaussian statistical distribution. Solute band shapes are perturbed by extracolumn volumes, nonideal retention processes and strong adsorptive interactions as they move from inlet to column to detector.

Choices of available stationary phases for gas chromatography (GC) have been fairly constant for many years. The same basic types of columns that do analogous separations can be obtained from any of a large number of sources, worldwide. Recent research has indicated that unique new substances have been developed that will play an important role in GC column technology.

Cancer can, in many cases, be cured if detected at an early, organ-confined stage. To help with early detection, there are considerable efforts to develop new potential biomarkers that improve current diagnosis and prognosis methods for different diseases.

Each week I get emails from various readers with questions or problems (see contact information at the end of this column). I enjoy most of these and often they give me fodder for one of these "LC Troubleshooting" columns.

Ionization suppression is a complex phenomenon associated with atmospheric pressure-based ionization techniques such as electrospray ionization (ESI) that affects mass spectrometry (MS) detection (1–4).

I first discovered the promised potential of lab-on-a-chip (LOC) technology at a Gordon Conference in the mid-1970s, when gas chromatography (GC) columns were reported on glass microscope slides. Since then, the technology's potential has fueled much innovation and product development. Yet this potential has not for the most part transmuted into practical, commercially available products.

I recently received an e-mail inquiry from a reader, along with the two chromatograms shown in Figure 1. Although not explicitly stated in the e-mail, it was clear that a debate was raging about how to best integrate this group of peaks.

The description of a chromatographic procedure in a US Pharmacopeia (USP) monograph contains necessary information that enables the procedure to be reproduced in a laboratory and typically includes system suitability requirements.

Arjunarishta (Parthadyarishta) is an important Ayurvedic formulation used for cardiovascular disorders and is prepared by fermenting the decoction of specified plant materials using flowers of Woodfordia fruticosa.

During the past few years, virus-like particles gained more and more attention as a new, safe class of vaccines, suitable for the prevention of virus induced infectious diseases (1) and for the therapeutical treatment of chronic diseases (2) and drug addiction (3).