HPLC and GC Articles

hplc chromatography and gc chromatography articles from pharmaceutical journals

Pinning Down Tailing Peaks, Part 2: Quantitative Measurement

In the world of practical chromatography, peak shapes only approach an ideal Gaussian statistical distribution. Solute band shapes are perturbed by extracolumn volumes, nonideal retention processes and strong adsorptive interactions as they move from inlet to column to detector.

Author(s): 
JOHN V. HINSHAW
Journal: 
LCGC Europe, Oct 1, 2009

The Advent and Potential Impact of Ionic Liquid Stationary Phases in GC and GCxGC

Choices of available stationary phases for gas chromatography (GC) have been fairly constant for many years. The same basic types of columns that do analogous separations can be obtained from any of a large number of sources, worldwide. Recent research has indicated that unique new substances have been developed that will play an important role in GC column technology.

Author(s): 
DANIEL W. ARMSTRONG , THARANGA PAYAGALA , LEONARD M. SIDISKY
Journal: 
LCGC Europe, Oct 1, 2009

Advanced Profiling Method based on MELDI–MS For High-Throughput Pattern Analysis in Proteomics

Cancer can, in many cases, be cured if detected at an early, organ-confined stage. To help with early detection, there are considerable efforts to develop new potential biomarkers that improve current diagnosis and prognosis methods for different diseases.

Author(s): 
CHRISTIAN W. HUCK , DOUGLAS T. GJERDE , GÜNTHER K. BONN
Journal: 
LCGC Europe, Oct 1, 2009

Too Many Changes

Each week I get emails from various readers with questions or problems (see contact information at the end of this column). I enjoy most of these and often they give me fodder for one of these "LC Troubleshooting" columns.

Author(s): 
JOHN W. DOLAN
Journal: 
LCGC Europe, Oct 1, 2009

Review of Volatile Perfluorocarboxylic Acids as Ion Pair Reagents in LC: Part II

Ionization suppression is a complex phenomenon associated with atmospheric pressure-based ionization techniques such as electrospray ionization (ESI) that affects mass spectrometry (MS) detection (1–4).

Author(s): 
WAYNE O. ARUDA , KENNETH O. ARUDA
Journal: 
LCGC North America, Oct 1, 2009

The Reality of Lab-on-a-Chip Technology for the Mass Spectrometry Laboratory

I first discovered the promised potential of lab-on-a-chip (LOC) technology at a Gordon Conference in the mid-1970s, when gas chromatography (GC) columns were reported on glass microscope slides. Since then, the technology's potential has fueled much innovation and product development. Yet this potential has not for the most part transmuted into practical, commercially available products.

Author(s): 
JACK HENION
Journal: 
LCGC North America, Oct 1, 2009

Integration Problems

I recently received an e-mail inquiry from a reader, along with the two chromatograms shown in Figure 1. Although not explicitly stated in the e-mail, it was clear that a debate was raging about how to best integrate this group of peaks.

Author(s): 
JOHN W. DOLAN
Journal: 
LCGC North America, Oct 1, 2009

Adjustment to the Chromatographic System in U.S. Pharmacopeia Monographs: Flexibility and Limitations

The description of a chromatographic procedure in a US Pharmacopeia (USP) monograph contains necessary information that enables the procedure to be reproduced in a laboratory and typically includes system suitability requirements.

Author(s): 
RAVI RAVICHANDRAN , HORACIO N. PAPPA
Journal: 
LCGC North America, Oct 1, 2009

HPLC Analysis and Standardization of Arjunarishta – An Ayurvedic Cardioprotective Formulation

Arjunarishta (Parthadyarishta) is an important Ayurvedic formulation used for cardiovascular disorders and is prepared by fermenting the decoction of specified plant materials using flowers of Woodfordia fruticosa.

Author(s): 
Uma R. Lal, Shailendra M. Tripathi, Sanjay M. Jachak, Kamlesh K. Bhutani, Inder P. Singh
Journal: 
Sci. Pharm. 2009; 77: 605–616

Asymmetrical Flow FFF as an Analytical Tool for the Investigation of the Physical Stability of Virus-Like Particles

During the past few years, virus-like particles gained more and more attention as a new, safe class of vaccines, suitable for the prevention of virus induced infectious diseases (1) and for the therapeutical treatment of chronic diseases (2) and drug addiction (3).

Author(s): 
R. Lang , L. Vogt , A. Zürcher , G. Winter
Journal: 
LCGC North America, Sep 1, 2009
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