High performance liquid chromatographic determination of aspirin and clopidogrel in tablets

A reverse phase high performance liquid chromatographic method to determine aspirin and clopidogrel in combined dosage form is proposed. The chromatographic resolution of aspirin and clopidogrel was obtained in a mobile phase consisting of 0.1% v/v triethylamine (pH 4.0):acetonitrile in the ratio 25:75% (v/v) in an isocratic elution. A detection wavelength of 225 nm and flow rate of 1 ml/min was used in the study. Nimesulide (20 µg/ml) was used as an internal standard. The system suitability procedures as precision, accuracy, LOD, LOQ, number of theoretical plates and tailing factor were studied.