HPLC and GC Articles

One of my roles as "LC Troubleshooting" editor is to field questions from readers about various problems with liquid chromatography (LC) separations. I am happy to provide answers, usually by e-mail (John.Dolan@LCResources.com

A simple, rapid, precise and accurate gas liquid chromatographic method was developed for the determination of ethanol in marketed preparation of abhayarishta using flame ionization detector. The separation was performed employing carbowax 20 M stainless steel column using nitrogen as a carrier gas at optimized conditions.

A new simple, sensitive and precise high performance thin layer chromatographic method has been developed for estimation of paracetamol and valdecoxib simultaneously from a combined dosage form.

A new simple, sensitive and precise high performance thin layer chromatographic method has been developed for estimation of paracetamol and valdecoxib simultaneously from a combined dosage form.

A simple, selective, rapid, precise and economical reverse phase HPLC method has been developed for the simultaneous estimation of paracetamol and aceclofenac from pharmaceutical dosage forms. The method was carried out on a Hichrom C18 (25 cm×4.6 mm i.d., 5 µ) column with a mobile phase consisting of acetonitrile:20 mM phosphate buffer (pH 5.0) (60:40 v/v) at a flow rate of 0.8 ml/min.

A reverse phase high performance liquid chromatographic method to determine aspirin and clopidogrel in combined dosage form is proposed. The chromatographic resolution of aspirin and clopidogrel was obtained in a mobile phase consisting of 0.1% v/v triethylamine (pH 4.0):acetonitrile in the ratio 25:75% (v/v) in an isocratic elution.

The objective of the present work is to develop a simple and sensitive method for analysis of human insulin injection by using reverse-phase high performance liquid chromatography technique. A reverse-phase high performance liquid chromatography method with UV-detection at room temperature has been developed for the analysis of insulin from formulation.

Polybrominated diphenyl ethers (PBDEs) have emerged as a significant class of persistent organic pollutants (POPs). These POPs join the organochlorine pesticides (OCs) and polychlorinated biphenyl (PCB) and polybrominated (PBB) biphenyl congeners as environmentally significant POPs that must be biomonitored continuously.

While previous "Validation Viewpoint" columns have focussed on conventional and recombinant pharmaceutical products, and at times, bioanalytical methods, we think it is also appropriate to address method validation as it relates to -omics-type studies.

Many gas chromatographers are considering hydrogen carrier gas instead of helium or nitrogen, but they might hesitate to do so because they have questions about performance, safety, or cost. This month's "GC Connections" focuses on these and other issues related to hydrogen in the gas chromatography laboratory.

Performance