Analysis of Acrylamide in Food by GC–MS

A positive chemical ionization GC–MS method for the analysis of acrylamide monomer in foods will be described and future areas for development offered. The method had a limit of detection of 5 ppb and was found to show good linearity to 1000 ppb. PCI SIM using ammonia as a reagent gas provided the best blend of sensitivity and selectivity.

Introduction :
To adequately assess acrylamide risk to humans, food levels need to be accurately measured and compiled. This prompts the need for development of analytical methods for extraction and quantification of acrylamide. The United States Food and Drug Administration (FDA) has published an LC–MS/MS method for acrylamide, and others have also published methods using either GC, GC–MS or LC–MS.7,8 Published detection limits for acrylamide in food range from 10-50 ppb depending on instrumentation. Here, the Trace DSQ™ is used to analyse acrylamide with linear dynamic range extending from 5-1000 ppb.
Experimental Conditions :
One gram of unknown sample and 100 µL of 13C3-Acrylamide solution (2000 ng/mL) were combined in 10 mL water. Standards were prepared in water as well, with 100 µL internal standard

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