Nanoflow LC–Q-TOF MS for De Novo Peptide Sequencing in Microbial Proteomics

The publication of several genome sequences has dramatically changed the scope of biochemical sciences. The availability of the libraries of genes that an organism can use to develop and to adapt to external stress has shifted biochemical research to the analysis of the final gene products; that is, proteins, their posttranslational modifications and their interactions with other biomolecules. The first step in this ‘proteomics’1 approach is the identification of proteins involved in a particular biological process. This work is generally based on so-called differential expression analysis, which generates information about genes that are switched on or off in the presence of a particular external signal.

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