NANO PARTICLE BASED STEM CELLS....

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Magnetic nano particles for Labeling Stem Cells

* An important aspect of successful cell transplantation is tracking and the monitoring of the grafted cells in transplant recipient.(1)

*To the screen cells in vivo,many techniques have been described using the nanoparticles (like quantum dots, pebbles and super paramagnetic iron-oxide [SPIO] nanoparticles), but exclusively SPIO nanoparticles are visualized by MRI are suitable in human medicine. SPIO nanoparticles are the negative contrast agents that will selectively shorten T2-relaxation time and hence, can be detected in tissue as a hypointense (which means dark) signal. MRI, as noninvasive method, may be then used not only to evaluate whether the cells have been successfully engrafted, but also to monitor the time course of cell migration and also their survival in targeted tissue.(1)

*This information may further help in order to optimize the process of transplantation procedure in terms of the number of the required cells, the method or site of cell administration and also the therapeutic time window after injury during which transplantation will be most effective.(1)

* Super paramagnetic iron-oxide nanoparticles usually consist of a crystalline iron oxide core and a polymer shell.(1)

*In order to prevent the aggregation, dextran is most commonly used surface coating.Dextran-coated SPIO (Super paramagnetic iron-oxide )ferumoxide nanoparticles are commercially available and have been approved as contrast agents by US FDA as Feridex(r) and Endorem(r) (or) as ultrasmall SPIO nanoparticles (Sinerem(r), Combidex(r)).(1)

* To facilitate uptake of nanoparticles into the cells, a common labeling approach is to combine the commercially available contrast agents and also transfection agents (for ex: Superfect, poly-L-lysine, Fugene(r) or protamine Lipofectamine,).(1)

*The advantage of the Endorem, as well as of carboxydextran-coated ferucarbotran is that they have been shown to be suitable contrast agents for the purpose of labelling rat or human MSCs ,ESCs and OECs, and don't require use of an transfection agent, however, they have a lower labeling efficiency than newly developed coatings. many strategies for the purpose of optimizing the delivery of the magnetic nanoparticles into cells are developed, like specific targeting and also the process of endocytosis of nanoparticles through the use of the transferring receptors, magnetodendrimers, transduction agents such as HIV-derived TAT protein or by use of electroporation.(1)

diagram(2)

magnetic nano particles

References:

1. http://www.medscape.com/viewarticle/715263_3

(accessed on 22nd October,2011:11.05 PM)

2. http://img.medscape.com/article/715/263/715263-fig1.jpg (accessed on 22nd October,2011:11.05 PM)

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Satyajeeth Pandey's picture

Dear Srravya A good work once you came back from your exams felt good when you said you did well in your exams all the best

Satyajit Panda Asst. professor Maharajah's College Of Pharmacy

Sandhya Sravya malla's picture

thank u very much for your valuable comment... m.sandhyasravya@gmail.com PHARMA WARRIORS
Sravani kompella's picture

hello sravya, nice blog by you... Could you please explain me regarding the pictures which you kept in the blog??
Sandhya Sravya malla's picture

by the way here is the answer to the question posed by u. MRI tracking of the SPIO-labelled stem cells. (A) Scheme of iron nanoparticle. The contrasting agent is Endorem(r) and it consists of a superparamagnetic ferrous oxide Fe3O4 core and is coated by a dextran shell. (B) Rat stem cell of mesenchyma (MSC) culture labelled with super paramagnetic iron-oxide nanoparticles and is stained as Prussian blue. (C) Transmission-electron micrograph of cluster of iron nanoparticles that are surrounded by acell membrane. (D) Cortical photochemical lesion that is visible on MRI 2 weeks later induction as a hyperintensive area. (E) Both cell implant (MSCs in the hemisphere contra lateral to that of the lesion) and lesion is hypointensive (dark) 2 weeks later implantation. (F) A few cells that are weakly stained for the Prussian blue were found in photochemical lesion in animals without the implanted cells. (G & H) A hypointensive signal in lesion was seen 7 days later to the IV injection of Endorem-labeled rat MSCs (I) Massive invasion of rat's SCs (Prussian blue staining counterstained with hematoxylin) into photochemical lesion 7 weeks later IV injection. (J) Longitudinal section of rat's spinal cord compresed lesion on MRI 5 weeks later compression. The lesion is seen as a hyperintensive area. (K) Prussian blue stain of a spinal cord compressed lesion (control animal in the procedure). (L) Longitudinal MRI of spinal cord compressed lesion populated with an IV injected nanoparticle-labeled MSCs 4 weekslater implantation. The lesion with nanoparticle-labeled cells is visualised as a dark hypointensive area . (M) Prussian blue stain of spinal cord lesion with IV injected nanoparticle-labeled stem cells m.sandhyasravya@gmail.com PHARMA WARRIORS
Siriki Praveen Kumar's picture

good blog presented very cleanly..

Regards,

S. Praveen Kumar.

sirikipraveen11@gmail.com

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Sandhya Sravya malla's picture

thank u very much......... m.sandhyasravya@gmail.com

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