Importance of single cell culture

Single cell culture is very important for the fundamental and mutation studies and it has a wide industrial application.
i.Single cell culture can be used successfully to obtain single cell clones.

ii.Plants can be regenerated from the callus tissue derived from the single cell clones.

iii.The occurrence of high degree of spontaneous variability in the cultured tissue and their exploitation through single cell culture are very important in crop improvement programmes.

iv.Isolated single cells can be handled as a microbial system for the treatment of mutagens and for mutant selection. Many cell lines resistant to amino acid analogues, antibiotics, herbicides, fungal toxins etc. have been selected by the simplest method.

v.Single cell culture in large scale could become a valuable technique for industrial production of such and important natural compound.

vi.Biotransformation means the cellular conversion of an exogenously supplied substrate compound not available in the cell or the precursor of a particular cellular compound to a new compound to a new compound or the known compounds in higher amounts.

References:
•Plant tissue culture by Kalyan Kumar
•Plant Biotechnology by Vas and Dikshit
•Pharmacognosy by C.K.Kokate et al
•Pharmacognosy by Trease and Evans