Applications Of Noe

4. Applications Of Noe:

Quantitatively the biggest use of the NOE is in determining the structures of biomolecules. A typical protein structure  determination requires the assignment and quantitation of approximately 2000 NOEs. This is usually done from 3D and sometimes 4D heteronuclear-edited NOSEY spectra, and increasingly the picking of peaks and their assignment is done automatically, with increasingly sophisticated algorithms to account for the inevitable wrong assignments and consequently structural distortions.