Ovarian Tissue Cryopreservation : in Ovary Cancer

After transplantation of ovarian tissue to women,To check the ovulation function regular Urine test carried out. In addition to that some drug therapy carried out :
1. Leuprolide acetate (Lupron), an injectable drug that blocks secretions of the pituitary gland, thereby optimizing the number of oocytes retrieved;
2. Human menopausal gonadotropin (Pergonal or hMG) or Follicle Stimulating Hormone (Metrodin or FSH), hormones that stimulate ovarian activity, are injected daily for about 6-10 days prior to the procedure;
3. Human chorionic gonadotropin (hCG), a hormone that mimics the action of the hormone which naturally induces ovulation, is injected 34 to 36 hours before retrieval and may be used after retrieval to supplement natural progesterone production;
4. Progesterone, a natural hormone that enables the uterus to support pregnancy, may be used as a daily injection after egg retrieval
These therapy used to mainly restore the normal function of implants.
Thank you
Pankaj Patel

very good question
i would like to give some outline which are most important then other approaches.

Ovarian cancer is the 3rd common gynecologic cancer that accounts for 32% of all gynecologic malignancies.
It causes 55% of all gynecologic cancer deaths. It is the 4th leading cause of cancer deaths in females.
Ovarian tissue collection and cryopreservation has been developed for patients who are facing the possible loss of their ovarian function and / or oocyte quality due to various medical conditions or necessary medical therapies.

At present the two main approaches are growth and development of the follicles in vitro or transplantation of the tissue back into the patient or into a host. Although the results seen so far are encouraging, very few pregnancies have been initiated with post-thaw ovarian tissue. The first birth following transplant of frozen ovarian tissue was reported in September 2004.

“Chemotherapy no more a bar for motherhood”

For thousands of young women cancer patients in India, the dream of becoming a mother remains what is essentially a dream. Their ovarian function is completely destroyed as they are immediately put on chemotherapy or radiotherapy.
“chemotherapy and radio therapy have a very negative effect on the ovaries. It destroys healthy cell and primordial follicles in the ovaries. As a result women are unable to conceive after treatment. So it protects ovary from the dangerous side effect of cancer chemotherapy.
When the patient turns disease free these ovarian cell back in their body.
For Conclusion from above data see my Conclusion Slide.
Thankyou
PANKAJ PATEL

Cryopreservation of red blood cells is done to store rare units, usually for up to 10 years. Truly rare units may be kept even longer. The cells are incubated in a glycerol solution which acts as a cryoprotectant ("antifreeze") within the cells. The units are then placed in special sterile containers in a freezer at very cold temperatures. The exact temperature depends on the glycerol concentration. Another Method for storage generally stored at 4° C.
Erythrocytes stored in an acid-citrate-dextrose preservative (ACD) at 4 to 7° C. undergo morphological and biochemical alterations. The cell becomes more sphere-shaped with an associated increase in osmotic and mechanical fragilities, the
glycolytic rate and organic phosphate compounds are decreased, inorganic phosphate is increased,and the differential between cell potassium and sodium is decreased.

On other side,
The respirocyte is a bloodborne 1-micron-diameter spherical nanomedical device designed by Robert A. Freitas Jr.,
Respirocytes are hypothetical, artificial red blood cells that can supplement or replace the function of much of the human body's normal respiratory system.

Thankyou
Pankaj patel

Oocyte test for detection of tumor promoting compounds : United States Patent 4983527
A unique, quick, inexpensive and highly accurate assay for detecting the presence of tumor promoters based on a measurable set of changes in amphibian oocytes in the presence of a tumor promoter. A secondary assay based on the protein released by the oocytes is employed to confirm the initial assay and quantify the potency of the tumor promoter present.
For more information refers : http://www.freepatentsonline.com/4983527.html
the second question i cant understand so please explain it properly to ease for understanding.
Thank you
Pankaj Patel

hi roshan
as u ask about brief data relates to recent development as given in my presentation given below :
At present the two main approaches are growth and development of the follicles in vitro or transplantation of the tissue back into the patient or into a host. Although the results seen so far are encouraging, very few pregnancies have been initiated with post-thaw ovarian tissue. The first birth following transplant of frozen ovarian tissue was reported in September 2004.
The technology is still considered “experimental”, and as such there is no guarantee of successful recovery of the tissue from storage. Much research is occurring worldwide, especially in the area of thawing the tissue and methods to stimulate mature follicle formation.

Development of experimental therapies:
Recently there are trial of cryopreservation of oocytes, embryos, and ovarian tissues to preserve ovarian function in such patients.
Inspite of the mentioned efforts ovarian cancer still has the worst prognosis among all gynecologic cancers.
Thankyou
Pankaj Patel

Dear sir,
As your question related to IVF-process process by which egg cells are fertilised by sperm outside the womb,( in vitro),and treatment of infertility.
Four to five medications normally are given :
1. Leuprolide acetate (Lupron), an injectable drug that blocks secretions of the pituitary gland, thereby optimizing the number of oocytes retrieved;
2. Human menopausal gonadotropin (Pergonal or hMG) or Follicle Stimulating Hormone (Metrodin or FSH), hormones that stimulate ovarian activity, are injected daily for about 6-10 days prior to the procedure;
3. Human chorionic gonadotropin (hCG), a hormone that mimics the action of the hormone which naturally induces ovulation, is injected 34 to 36 hours before retrieval and may be used after retrieval to supplement natural progesterone production;
4. Progesterone, a natural hormone that enables the uterus to support pregnancy, may be used as a daily injection after egg retrieval
These therapy used to mainly restore the normal function of implants.

Polycystic ovary syndrome (PCOS) is an endocrine disorder,
common hormonal disorder among women of reproductive age, and is a leading cause of infertility.
Yes, By cryopreservation, eggs, sperm and reproductive tissue can be preserved for later IVF the transfer later to the patient.
Pankaj patel
NMiMS-Shirpur

hi
For transportation of such cryopreserved tisue mostly Liquid Nitrogen is used other then this DMSO, ethyleneglycol,glycerol used as cryoprotectant.these whole process also called as "Cold Chain".
Thawing means Melting-But in case of these approach Thawing means the cyopreserved tissue warmed to Body temperature,these done to ensure its normal functioning before transplantation.
In addition to these the Risks associated with cryopreservation are as below :
Dehydration,
Ice-formation (intracellular/extracellular),
Solution effect.
Thankyou
Pankaj Patel

Thnkyou
1.Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as 77 K or −196 °C.like other types of preservation of sperms and stem cell these whole proces carried out. There is No any disrupt of cellular damage due to this.

2.After transplantation suufiend amt os nutrients and other tissue activing drugs also giving to the patient. As the transplatatuion done the circulation starts but The Full function ofthese approach checked timely to ensure the successful transplantation.

3.Yes, Vertificatin is the newer method to provide the benefits of cryopreservation without damage due to ice crystal formation, in techniques that are far superior to freezing.

hi hemangi
thankyou for asking your query.
There are so many guidelines for Cryopreservation for reproductive tissue:

-Material should only be stored until the age at which it is "acceptable" to be used for the achieving pregnancy
-That consideration should be given to the fate of store material should it be required to be used posthumously
-In the case of children stored material should be discarded on the death of the provider and the parents have no right to its usage
-In the case of single adults and couples posthumous donation (sperm) is possible with prior consent from the provider
-Posthumous donation of oocytes is not recommended
-Stored material may be used for research after the provider has received adequate counselling for the implications of this choice
-Donation of reproductive material should only occur when the donor was healthy at time of freezing
-The entire healthcare team should be involved in the decision making and may involve the oncologist, reproductive specialist, psychologist/counsellor etc.

The Deccan Fertility Clinic and Keyhole Surgery Center is India's first budget IVF clinic and an ISO 9001-2000 certified center,there are also somany cneters for that type of research other than this.

hi ritesh
as ovarian tissue Cryopreservation similar to that of Sperm, stem cell preservation.
after that when the necessary situation this all reimplanted to host to fulfill the requirement.
so, cryopreservation is preliminary stage to that of implantation.
thank you

hi Bhawna
Freezable tissues:(using cryopreservation)
Semen,Blood (special cells for transfusion, or stem cells),Tissue samples like tumors and histological cross sections,* Human eggs (oocytes),Human embryos,Ovarian tissue.
Mainly freezing condition maintain to to the all biological activity.Tissues are preserved by cooling to low sub-zero temperatures, such as 77 K or −196 °C .
THANK YOU

hi komal
thanks
the solution for transportation mainly Nitrogen is used which stored or secured it by freezing at -200 C.

at this freezing condition no any external environmental effect on ovarian tissue.

hi komal
in case of storage all the tissue not remains same positive results.
some of them might be loss thier activity. so the ratio of success is quit low. and as this process under developing stage further research also going on this direction to retain preserved tissue activity.
thanks

IVF-pregnancy required for some couples struggling with infertility. In some cases, such as uterus or fallopian tube abnormalities, surgery may be required before any IVF procedures are attempted.
To make success for trasplantation :
Four to five medications normally are given:
1. Leuprolide acetate (Lupron), an injectable drug that blocks secretions of the pituitary gland, thereby optimizing the number of oocytes retrieved;
2. Human menopausal gonadotropin (Pergonal or hMG) or Follicle Stimulating Hormone (Metrodin or FSH), hormones that stimulate ovarian activity, are injected daily for about 6-10 days prior to the procedure;
3. Human chorionic gonadotropin (hCG), a hormone that mimics the action of the hormone which naturally induces ovulation, is injected 34 to 36 hours before retrieval and may be used after retrieval to supplement natural progesterone production;
4. Progesterone, a natural hormone that enables the uterus to support pregnancy, may be used as a daily injection after egg retrieval
These therapy used to mainly restore the normal function of implants.

With regards
Pankaj Patel
M.Pharm
NMiMS-Shirpur

Dear sir,
there are so many problems-
There are some risks associated with the laparoscopy, and adverse outcomes are seen in less than 1% of all cases.
It is possible that when the tissue is thawed it may not yield usable oocytes, or may not result in pregnancy.
The process of freezing the tissue or its prolonged storage may result in some damage to the immature follicles.
in addition to ALL THIS No guaranty fot the success of these therapy means success ration is quit less.
In addition to this risks associated with Cryopreservation are:Solution effect, Ice formation, dehyration.

In india the most common problem related to Transportation of these cryopreserved tissue, "Cold Chain" Broad term used for these approach.
With Regards
Pankaj Patel

thnks suhaas
1.Glycerol and DMSO have been used for preservation of sperm, ovarian tissue and embryos can also cold-preserved in liquid nitrogen.
2.After the cancer therapy or complete recovery from diseased condition these preserved tissue implanted back.
3.No,this approach not ruled out possibility of cancer.
4.there may be possibility but not completely guaranteed for that though this approach under development.
Thankyou

thanks siddh

in India General Dr Naresh Kumar, commandant, Army Hospital
Dr Pankaj Talwar, IVF specialist, Army Hospital-crried out this research.
Though there is no guarantee for the success of this but the first case for birth using this happened on sep. 2004
for more detail please refer reference of timesofindia mumbai.

thankyou mam
ovarian cancer sign and symptoms are.......
not cause obvious symptoms. But, as the cancer grows, symptoms may include:
Pressure or pain in the abdomen, pelvis, back, or legs
A swollen or bloated abdomen
Nausea, indigestion, gas, constipation, or diarrhea
feeling very tired all the time
Less common symptoms include:
Shortness of breath
feeling the need to urinate often
unusual vaginal bleeding (heavy periods, or bleeding after menopause)
Most often these symptoms are not due to cancer, but only a doctor can tell for sure. Any woman with these symptoms should tell her doctor.

RISK FACTORS :Family history of cancer,Personal history of cancer,Personal history of cancer,Menopausal hormone therapy.
in above cases these therapy utilized means your statement is now true in above cases..
THANK YOU

hi varsha

it is possible to make bank like sperm bank.
if women not having physiological defect related to ovary, and if all criteria related to immune system satisfied then this can be possible to transfer ovary cell as you says.
THANK YOU

thakyou hussain
methods like for ptient with cancer-
Human secondary follicles were isolated from ovarian tissues obtained from cancer patients and grown in vitro within a bio-engineered culture system for 30 days.
other methods which are under study on animals-like culture of pieces of bovine ovarian cortex; in this situation almost all primordial follicles in the cortex activate, and other like drafting ovarian cortical pieces beneath the chorio-allantoic membrane (CAM) of chick embryos; in this situation follicle growth arrest is maintained. Such other so many studies under development on animal.

as these process under development No guarantee for success of these therapy but the results came during these are interesting.
in 2004 September there is the first successful case for ovary tissue transplantation for Birth of Baby.
for more information see slides.

thnks brother
if women having problem related to Ovary from birth then it cant be possible to use this therapy.
in case if ovary lost the ovulation formation capacity deu to certain therapy or physiological conditions then only its possible to use these therapy considering criteria as same as organ or tissue transplantation.