sirisha's blog

GENOMICS!!!!

Hello everybody, As listed in the topics of the month, I'm here to discuss about GENOMICS. Entirely new scenario in science began with the invention of Microscope, later cell, Organelles and finally major landmark is the discovery of DNA. Better understanding of genetic information was started in 1953 with its discovery.

NORTHERN BLOTTING TECHNIQUE

Hi everyone..as listed in the topics, i am here to present Northern blotting technique. Well, the northern blotting technique is similar to Southern blotting but the major difference is that the present technique deals with differentiating the gel fractionated segments of RNA. As said before, blotting is entrapment of nucleic acid on to Nitrocellulose or Nylon membrane.

SOUTHERN BLOTTING TECHNIQUE!

Southern Blotting Technique: It is named after its originator. Let us deal what exactly it says. Blotting is a process of transferring the fragments in a gel to a membrane made of Nitrocellulose or Nylon. Procedure: 1. The DNA is cut with a suitable Restriction Endonucleases and run on a gel. 2. The DNA is denatured by treatment with an alkali (NaOH).

SOMNILOQUY!!!

In our daily lives, we find many people with different attitudes and behaviour. Some people enjoy themselves and let others. While some stay away from the social group. Some others lie in the mid of these two groups. At this stage of life, we spend much of our happy moments in chit chatting with friends. Long goes the stories.,,,no intermissions.. the sagas continue..Just talking talking!! While talking in unusual times is considered as a disorder!! That's what is SOMNILOQUY! It is a disorder characterized by talking during sleep. WHEN DOES IT OCCUR??[1]

INDIA AIMING HIGH!!!!

Let me start with two stories ending on a similar note. People often say we should change according to time. Else we carry the "old cat" tag or tykes say its "generation gap". I don't define generation gap with regard to age but with time. But sometimes we find our goals or thinking ways similar to elders may be of decade's gap.

AUDIO BLOG - LAC OPERON CONCEPT

Hello everyone..We have reached the ending phase of this hot scorching summer and i hope you all had a great time in these holidays. (:
Coming to my audio topic - The Lac Operon Concept.

[swf file="http://c450871.r71.cf2.rackcdn.com/LacOperonConceptSirishaPingaliMay2011..."]

The theory was first proposed by French Scientists Francois Jacob and Jacques Monod in the year 1961. They were awarded Nobel Prize in 1965 for their contribution.

DNA SEQUENCING.. A VERY LONG STORY!!!

Hello all, I am here to present an important test which gained importance recently viz. DNA Sequencing Procedures followed: Maxam and Gilbert Method & Sangers method. Basic principle involved in either method: Series of DNA fragments are generated with a common starting point but with variable termini. Illustration by Sangers method

WINE FOR ALL OCCASIONS!!!!

An ideal victory may be getting a gold medal in studies, or holding a world cup just like our men in blue did few days back or winning a F1 race etc. All these have a common task which is none other than the Celebrations!!! Thanks to Cardbury advert for evoking the "Celebrations chocolates" which have been nonpareil gift particularly liked by children (you know - Kuch mitha ho jaaye :))

Pages